Laboratory of Orthopedic Research
Orthopedics is the branch of medicine concerned with diseases, injuries and conditions of the musculoskeletal system, or the body’s muscles and skeleton, and including the joints, ligaments, tendons, and nerves.
Orthopedic surgery is made up of physicians and other healthcare professionals who provide comprehensive orthopedic services. Their expertise provides treatment and care of diseases, injuries, fractures, and pain. Orthopedists also design rehabilitation programs for the disabled and participate in ongoing musculoskeletal research.
Dr. Grande's research aims to improve treatments for injuries of the musculoskeletal system, including cartilage regeneration and repair, meniscus repair, tendon repair, and bone fracture augmentation and fusion. Dr. Grande is widely recognized as a world leader in cartilage repair. He pioneered the first cell-based therapy for articular cartilage repair, studies that led to the commercialization of cartilage transplantation for the current surgical treatment of focal cartilage defects in clinical use today, called autologous chondrocytes transplantation.
Recently, he and his collaborators have been developing novel cell-modified scaffolds for enhancing tendon repair as well as innovative methods to provide osteogenic and osteoconductive gene therapy and tissue-engineered constructs for providing abundant bone graft substitutes for bone injuries.
The lab recently received an instrument Instron 5566 material testing system. This will allow our students and residents to conduct strides in to determine the strength of various orthopedic constructs such as; fracture stiffness of bone, the strength of tendon repairs. The team is collaborating with Sleiman Ghoryeb, PhD, an engineer at Hofstra University. He will be examining the utility of ultrasound imaging for assessing cartilage repair.
The orthopaedic research lab has a number of projects currently ongoing that span the breadth of current orthopaedic topics; these include the following two main areas of focus but all are involved with regenerative medicine:
Tendon Regeneration: Common injuries of the tendon include the rotator cuff of the shoulder and flexor tendons of the hand. The ORL has ongoing projects in both and have developed methods to coat surgical sutures, with growth factors to locally deliver and augment the time to heal as well as the quality of repair.
Another recent discovery of the lab is that by inhibiting metalloprotease activity [an enzyme which results in degradation of collagen] in individuals who have recent tendon tears can result in improved repair and return to function.
Cartilage Repair: Our efforts now focus on how current clinical practice can be improved by providing the correct conditions for repair with true hyaline articular cartilage. These strategies include providing local delivery of growth factors for recruitment of stem cells to the defect site and then pushing their development toward a cartilage phenotype. Other strategies involve development of novel bioactive scaffolds that contain caches of specific growth factors to orchestrate the regenerative cascade.
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Name: Pasquale Razzano, MS |
Select Publications:
Grande DA, Breitbart AS, Mason J, Paulino C, Laser J, and R Schwartz: Cartilage Tissue Engineering: Current Limitations and Solutions, Clin. Orthop. Rel. Res., 367S, 176-185, 1999.
Guilak F., Grande DA, Zell RA, Erickson G., CT Rubin, McLeod KJ, Donahue HJ: “Mechanically Induced Calcium Waves in Articular Chondrocytes are Inhibited by Gadolinium and Amiloride”. J. Orthop. Res Vol. 17:421-429, 1999.
Breitbart AS, Grande DA, Mason JM, Barcia M, James T, and Grant RT: Gene Modified Tissue Engineering: Applications for Bone Healing using Cultured Periosteal Cells Retrovirally Transduced with BMP-7 Gene, Ann Plast Surg 42: 488-495, 1999.
Breitbart As, Mason JM, Urmacher C, Barcia M, Pergolizzi RG, Grant RT, Grande DA: Gene Enhanced Tissue Engineering: Applications for Wound Healing using Culured Dermal Fibroblasts Retrovirally Transduced with the PDGF-B Gene. Ann Plast Surg 43: 632-639, 1999.
Mason J, Grande DA, Barcia M, Grant R, Pergolizzi R, and A. Breitbart: Expression of Human Bone Morphogenic Protein 7 in Primary Rabbit Periostealells: Potential Utility in Gene Therapy for Osteochondral Repair. Gene Therapy, 5[8]:1098-1104, 1998.
Torzilli PA, Grande DA, and JM Arduino: Diffusive Properties of Immature Articular Cartilage. J. Biomedical Materials Research, 40, 132-138, 1998.
Breitbart AS, Grande DA, Kessler R, Ryaby JT, Fitzsimmons RJ, and Grant RT: Tissue Engineered Bone Repair of Calvarial Defects Using Cultured Periosteal Cells. Journal of Plastic and Reconstructive Surgery. 101, No.3, 567-574, 1998.
Kessler MW, Ackerman G, Dines JS, Grande DA: “Emerging Technologies and Fourth Generation Issues in Cartilage Repair”. Sports Medicine and Arthroscopy Review. 16 [4] pg 246, 2008.
Rosenbaum A, Grande DA, and Dines J: “The Use of Mesenchymal Stem Cells in Tissue Engineering” Organogenesis 4:1 23-27, 2008.
Kessler M and Grande DA: “Tissue Engineering and Cartilage” Organogenesis 4:1 29-32, 2008.
Dines JS, Grande DA, ElAttrache N, Dines DM.: “Suture Coating and Augmentation to Enhance Tendon Healing”. Techniques in Orthopaedics. 22(1):20-25, 2007.
Dines JS, Grande DA, Dines DM: “Tissue Engineering and Rotator Cuff Tendon Healing”. J Shoulder and Elbow Surgery. 16 S-204-207, 2007.
Dines JS, Weber L, Prajapati R, Bowman S, Razzano P, Bonassar L, Timmer M, Dines DM, Grande D: “GDF-5 Coated Sutures to Enhance Tendon Healing in a Rat Model”. J Shoulder and Elbow Surgery. 16 S215-221, 2007.
Leo A and D. Grande: “Mesenchymal Stem Cells in Tissue Engineering” Cells, Tissues, and Organs. 183, p.112-122. 2006.
Grande DA, Southerland SS, Manji R, Schwartz RE, and Lucas PA: Repair of Articular Cartilage Defects Using Mesenchymal Stem Cells. Tissue Engineering (1)4:345-353, 1995.
Freed LE, Grande D, Emmanuel J, Kwan M, Marquis JC, Lingbin Z, Dunkelman N, Langer R: Joint Resurfacing Using Allograft Chondrocytes and Synthetic Biodegradable Polymer Scaffolds. J. Biomed. Mat. Res. 28: 891-900, 1994.
Grande, D., Pitman, M., Peterson, L., Menche, D., and Klein, M: The Repair of Experimentally Produced Defects in Rabbit Articular Cartilage by Autologous Chondrocyte Transplantation. J. Orthop. Res. 7:208-214, 1989.