The Scientific Cores at The Feinstein Institute for Medical Research and the Cold Spring Harbor Laboratory (CSHL) Cancer Center’s Shared Resources have established a collaboration agreement for the reciprocal use of core facilities. Investigators from the Feinstein Institute will have access to all CSHL core services. This collaboration is based on a fee-for-service at internal rates and scientific collaborations. The partnership will help investigators to perform state-of-the-art research in all scientific programs.
The CSHL Cancer Center has nine shared resources that facilitate research with state-of-the-art technology and integral services. With the support of world-class staff, these core facilities ensure that researchers have all the necessary tools to make breakthrough discoveries.
The primary purpose of the Animal Shared Resource is to provide researchers with facilities for housing and maintaining laboratory animals. Experienced staff perform all aspects of animal husbandry and assist researchers with specialized procedures and protocol development.
The Animal & Tissue Imaging Shared Resource provides both expertise and access to state-of-the-art non-invasive imaging modalities, tissue imaging, and pathology. This Resource allows researchers to visualize a broad range of parameters without invasive or terminal procedures, obtain high quality tissue sections, and access extensive pathology services.
The Antibody & Phage Display Shared Resource provides a central site for the production of antibodies for research using a highly efficient and cost-effective method. This resource uses both phage display technology and classical hybridoma development protocols. The phage display branch of the shared resource provides the reagents and expertise to carry out the selection of Fabs (antibody fragments) against purified protein. This new technology is capable of rapidly producing high-affinity synthetic antibodies in a manner that complements existing expertise in classical hybridoma development and antibody production and purification. The shared resource maintains a library of all hybridomas generated, and will provide cell lines or antibody preparation from stored lines on request.
The Bioinformatics Shared Resource (BSR) provides essential services and technical support for Next Generation Sequencing analysis. From providing advice at the planning stages of data-intensive experiments to developing customized software tools for analyzing data, the BSR works closely with members to support research projects. The BSR is happy to guide members in using web-based interfaces to access sophisticated computational tools, enabling researchers to do much of the analysis on their own.
Recent advances in Cryo-Electron Microscopy (cryo-EM) have made it possible to visualize extremely complex biological systems, providing novel molecular insight into human biology and disease. Cryo-EM has become an essential structural biology tool, enabling the determination of 3D structures for large biological macromolecules and complexes, as well as cellular components. At CSHL, the Cyro-EM Facility offers researchers access to a cutting edge instrument that can be applied broadly to study macromolecular machines.
The DNA Sequencing Shared Resource provides access to high throughput Next Generation Sequencing technologies and to full-service Sanger DNA sequencing using fluorescence-based technology. These combined resources offer cutting-edge technology alongside convenient in-house services for a broad range of genetic analysis.
There is nothing more frustrating than running out of a Reagent in the middle of an experiment. The CSHL On-Site Freezer Program is fully stocked with more than 275+ commonly-used reagents from Agilent, Applied Biosystems, New England Biolabs & Roche. The reagents are available for purchase at substantial discounts with the convenience of same-day pick-up in two on-campus locations.
Flow Cytometry is an essential tool that is widely used in cancer research. The Flow Cytometry Shared Resource provides equipment, training, and operating assistance for cell sorting and analysis. Facility staff oversees equipment maintenance, trains new users, and assists with assay development and operation of equipment.
This Core provides cutting-edge technology to assay gene function both in living animals and in cell culture. The Functional Genomics and Genetics Shared Resource offers assistance with the construction and management of genetically altered mouse strains, as well as RNAi technologies and CRISPR reagents.
Technological advances open new venues for discovery in biology. The innovative Machine Shop at CSHL encompasses an in-house workshop for custom design and fabrication of scientific equipment. We work closely with investigators to provide custom solutions to engineering problems they may encounter, as well as to design and fabricate specialized equipment to advance their research.
The Mass Spectrometry Shared Resource provides services to elucidate the primary structure of novel proteins and to analyze protein/protein interactions, post-translational modifications and protein levels. The Resource also offers the ability to detect lipids, metabolites, and other small molecules. The equipment in the facility has been upgraded to provide state-of-the-art analysis using microcapillary LC-MS/MS on linear ion traps and Orbitraps, hybrid quadrupole-TOF mass spectrometers and MALDI-TOF.
The Microscopy Shared Resource provides core cellular imaging services for cancer researchers. It offers state-of-the-art microscopes, expert training, and an interactive environment for scientists to learn about the latest advancements in imaging technology. The Resource provides access to wide-field fluorescence, deconvolution, and confocal microscopy as well as live cell imaging, super-resolution structured illumination microscopy, and standard transmission and immuno-electron microscopy.
For many types of research, Tissue Culture offers the ideal model system: rapid, cost effective, and easy to genetically manipulate. The CSHL Tissue Culture Facility offers affordable access to hundred of cell lines, with many common stocks being actively cultured and readily available. Several specialized tissue culture rooms are available and staffed across campus to support the needs of researchers and provide training. In addition, mycoplasma testing and cell-line authentication is coordinated through this facility.