The Scientific Cores at The Feinstein Institute for Medical Research and the Cold Spring Harbor Laboratory (CSHL) Cancer Center’s Shared Resources have established a collaboration agreement for the reciprocal use of core facilities. Investigators from the Feinstein Institute will have access to all CSHL core services. This collaboration is based on a fee-for-service at internal rates and scientific collaborations. The partnership will help investigators to perform state-of-the-art research in all scientific programs.
The CSHL Cancer Center has nine shared resources that facilitate research with state-of-the-art technology and integral services. With the support of world-class staff, these core facilities ensure that researchers have all the necessary tools to make breakthrough discoveries.
The primary purpose of the Animal Shared Resource is to provide researchers with facilities for housing and maintaining laboratory animals. Experienced staff perform all aspects of animal husbandry and assist researchers with specialized procedures and protocol development.
The Animal & Tissue Imaging Shared Resource provides both expertise and access to state-of-the-art non-invasive imaging modalities, tissue imaging, and pathology. This Resource allows researchers to visualize a broad range of parameters without invasive or terminal procedures, obtain high quality tissue sections, and access extensive pathology services.
The Antibody & Phage Display Shared Resource provides a central site for the production of antibodies for research using a highly efficient and cost-effective method. This resource uses both phage display technology and classical hybridoma development protocols. The phage display branch of the shared resource provides the reagents and expertise to carry out the selection of Fabs (antibody fragments) against purified protein. This new technology is capable of rapidly producing high-affinity synthetic antibodies in a manner that complements existing expertise in classical hybridoma development and antibody production and purification. The shared resource maintains a library of all hybridomas generated, and will provide cell lines or antibody preparation from stored lines on request.
The Bioinformatics Shared Resource (BSR) provides essential services and technical support for Next Generation Sequencing analysis. From providing advice at the planning stages of data-intensive experiments to developing customized software tools for analyzing data, the BSR works closely with members to support research projects. The BSR is happy to guide members in using web-based interfaces to access sophisticated computational tools, enabling researchers to do much of the analysis on their own.
The DNA Sequencing Shared Resource provides access to high throughput Next Generation Sequencing technologies and to full-service Sanger DNA sequencing using fluorescence-based technology. These combined resources offer cutting-edge technology alongside convenient in-house services for a broad range of genetic analysis.
Flow Cytometry is an essential tool that is widely used in cancer research. The Flow Cytometry Shared Resource provides equipment, training, and operating assistance for cell sorting and analysis. Facility staff oversees equipment maintenance, trains new users, and assists with assay development and operation of equipment.
This Core provides cutting-edge technology to assay gene function both in living animals and in cell culture. The Functional Genomics and Genetics Shared Resource offers assistance with the construction and management of genetically altered mouse strains, as well as RNAi technologies and CRISPR reagents.
The Mass Spectrometry Shared Resource provides services to elucidate the primary structure of novel proteins and to analyze protein/protein interactions, post-translational modifications and protein levels. The Resource also offers the ability to detect lipids, metabolites, and other small molecules. The equipment in the facility has been upgraded to provide state-of-the-art analysis using microcapillary LC-MS/MS on linear ion traps and Orbitraps, hybrid quadrupole-TOF mass spectrometers and MALDI-TOF.
The Microscopy Shared Resource provides core cellular imaging services for cancer researchers. It offers state-of-the-art microscopes, expert training, and an interactive environment for scientists to learn about the latest advancements in imaging technology. The Resource provides access to wide-field fluorescence, deconvolution, and confocal microscopy as well as live cell imaging, super-resolution structured illumination microscopy, and standard transmission and immuno-electron microscopy.